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Blood, Vol. 105, Issue 2, 679-681, January 15, 2005
Vascular leukocytes contribute to tumor vascularization
Blood Conejo-Garcia et al.
105: 679
Supplemental materials for: Conejo-Garcia et al , Vol 105, Issue 2, 679-681
Files in this Data Supplement:
- Document 1.Supplementary methods and formation of functional blood vessels by tumor-infiltrating VLCs and HUVEC cells (PDF, 271 KB) -
The document describes the methodology used for the preparation of the specimens, sorting and real-time polymerase chain reaction (PCR). It also contains additional data from more experiments addressing the formation of blood vessels in Matrigel plugs by VLCs and HUVECs, as well as from a preliminary experiment that explored the presence of VLCs in normal mouse tissues. Images were viewed through a Nikon Eclipse E400 inverted microscope with 10×, 20×, and 40× objectives (Nikon, Tokyo, Japan). Images were acquired with a CoolSnap-Pro digital camera and Phase 3 Imaging software (Media Cybernetics, Silver Spring, MD).
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