|
|
Blood, Vol. 105, Issue 6, 2480-2486, March 15, 2005
Regulation of human auto- and alloreactive T cells by indoleamine 2,3-dioxygenase (IDO)–producing dendritic cells: too much ado about IDO?
Blood Terness et al.
105: 2480
Supplemental materials for: Terness et al, Vol 105, Issue 6, 2480-2486
Files in this Data Supplement:
- Figure S1. T-cell regulatory property of dendritic cells pretreated with TNF-α and IFN-γ. (PDF, 40 KB) -
Mature Dendritic Cells (mDCs) of 8 healthy donors, in which IDO synthesis was stimulated by an enhancer (1000U/ml TNF-
 ) in addition to IFN- (1000U/ml), were co-cultured at decreasing ratios with autologous anti-CD3-stimulated T cells for 3 days. The control consisted of mDCs without IDO-inducer. Proliferation was assessed by adding [3H]thymidine. Single values represent the percentage of maximum stimulation (=100%). The difference between the T-cell stimulatory capacity of native DCs and IDO-DCs was statistically not significant.
- Figure S2. Complete suppression of T-cell proliferation by dendritic cells pretreated with protein synthesis inhibitor. (PDF, 24 KB) -
Mature Dendritic Cells of 3 healthy donors were loaded with MBP, treated for 30 min. with a selective protein synthesis inhibitor (=PI; 100µg/ml) and extensively washed. Vital staining with subsequent FACS analysis showed unimpaired cell viability. PI-treated as well as untreated DCs were coincubated at a ratio of 1:10 with H. saimiri-transformed MBP-specific T cells (clone ES-BP8T). DCs and T cells shared at least one HLA-DR antigen. Controls consisted of MBP-DCs or T cells only. Cell proliferation was measured after 4 days by [3H]thymidine incorporation. Data represent mean $#177; SD and are expressed as a percentage of the positive control values (= 100%). PI-treated DCs completely suppress the T-cell response, showing that suppression below basic proliferation of transformed T cells is possible.
|
|
