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Blood, Vol. 105, Issue 4, 1508-1514, February 15, 2005
Tumor metastasis but not tumor growth is dependent on Src-mediated vascular permeability
Blood Criscuoli et al.
105: 1508
Supplemental materials for: Criscuoli et al, Vol 105, Issue 4, 1508-1514
Files in this Data Supplement:
- Figure S1. Reduced perivascular accumulation of fibrin/FDPs in 4C8 astrocytoma tumor growth in Src-deficient mice (JPG, 15 KB)
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Immunostaining of cryosections of a human astrocytoma (4C8) injected into the cortex of src+/- or src-/- mice backcrossed onto an immunodeficient Rag2-null background. Following stereotaxic tumor injection, tumors were grown for 3 weeks, and endogenous perivascular fibrin/FDPs and blood vessels were detected with anti-fibrinogen (green) and anti-CD31 antibodies (red). Bar = 50µm (A). Lower–magnification detection of 4C8 tumor-associated fibrin/FDPs was also performed with a horseradish peroxidase–conjugated secondary antibody to label an antifibrinogen primary antibody, followed by incubation with diaminobenzidine. Edge staining of the surface of the brain is nonspecific reactivity, in contrast to the blood vessel immunostaining in the brain tumor–injected cortex. Bar = 400µm (B). Micrographs were collected on either a laser scanning confocal microscope (A) or a phase microscope equipped with a CCD camera (B), as described in "Materials and methods."
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