|
|
Blood, Vol. 105, Issue 4, 1590-1597, February 15, 2005
Impaired dendritic-cell homing in vivo in the absence of Wiskott-Aldrich syndrome protein
Blood de Noronha et al.
105: 1590
Supplemental materials for: de Noronha et al, Vol 105, Issue 4, 1590-1597
Files in this Data Supplement:
- Movie 1A. Abnormal motility of WASp-deficient immature DCs (MOV file, 1.32 MB)
-
Time-lapse video microscopy showed that immature BMDCs derived from control mice moved rapidly across an FN-coated surface in a Dunn chamber (see "Materials and methods"). The BMDCs polarized, formed a clear leading edge, and established transient but stable contact at this end (Movie 1A). This was followed by retraction of the trailing edge and net forward motion. In contrast, WASp-null BMDCs failed to establish a dominant leading edge and were unable to detach appropriately (Movie 1B). This resulted in the formation of a relatively static hyperextended cell morphology, oscillation of the cell body between each pole, and restricted translocation.
- Movie 1B. Abnormal motility of WASp-deficient immature DCs (MOV file, 1.31 MB)
-
Time-lapse video microscopy showed that immature BMDCs derived from control mice moved rapidly across an FN-coated surface in a Dunn chamber (see "Materials and methods"). The BMDCs polarized, formed a clear leading edge, and established transient but stable contact at this end (Movie 1A). This was followed by retraction of the trailing edge and net forward motion. In contrast, WASp-null BMDCs failed to establish a dominant leading edge and were unable to detach appropriately (Movie 1B). This resulted in the formation of a relatively static hyperextended cell morphology, oscillation of the cell body between each pole, and restricted translocation.
- Movie 2A. Abrogated motility of WASp-deficient mature DCs in response to CCL21 (MOV file, 410 KB)
-
Time-lapse videomicroscopy showing response of LPS-matured DCs to CCL21 in a Dunn chamber (see "Materials and methods"). As expected, LPS-matured DCs derived from both normal and WASp-null animals migrated minimally in CM medium alone (Movie 2A-B). In the presence of CCL21, normal BMDCs increased their speed and distance, and traveled from their origin (Movie 2C), while WASp-null cells were significantly less motile (particularly in terms of persistence of migration) over this time period (Movie 2D).
- Movie 2B. Abrogated motility of WASp-deficient mature DCs in response to CCL21 (MOV file, 409 KB)
-
Time-lapse videomicroscopy showing response of LPS-matured DCs to CCL21 in a Dunn chamber (see "Materials and methods"). As expected, LPS-matured DCs derived from both normal and WASp-null animals migrated minimally in CM medium alone (Movie 2A-B). In the presence of CCL21, normal BMDCs increased their speed and distance, and traveled from their origin (Movie 2C), while WASp-null cells were significantly less motile (particularly in terms of persistence of migration) over this time period (Movie 2D).
- Movie 2C. Abrogated motility of WASp-deficient mature DCs in response to CCL21 (MOV file, 409 KB)
-
Time-lapse videomicroscopy showing response of LPS-matured DCs to CCL21 in a Dunn chamber (see "Materials and methods"). As expected, LPS-matured DCs derived from both normal and WASp-null animals migrated minimally in CM medium alone (Movie 2A-B). In the presence of CCL21, normal BMDCs increased their speed and distance, and traveled from their origin (Movie 2C), while WASp-null cells were significantly less motile (particularly in terms of persistence of migration) over this time period (Movie 2D).
- Movie 2D. Abrogated motility of WASp-deficient mature DCs in response to CCL21 (MOV file, 409 KB)
-
Time-lapse videomicroscopy showing response of LPS-matured DCs to CCL21 in a Dunn chamber (see "Materials and methods"). As expected, LPS-matured DCs derived from both normal and WASp-null animals migrated minimally in CM medium alone (Movie 2A-B). In the presence of CCL21, normal BMDCs increased their speed and distance, and traveled from their origin (Movie 2C), while WASp-null cells were significantly less motile (particularly in terms of persistence of migration) over this time period (Movie 2D).
|
|
