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Blood, Vol. 105, Issue 3, 1121-1126, February 1, 2005
FcRH1: an activation coreceptor on human B cells
Blood Leu et al.
105: 1121
Supplemental materials for: Leu et al, Vol 105, Issue 3, 1121-1126
Files in this Data Supplement:
- Figure S1. Analysis of human immunoglobulin binding to FcRH1 retroviral transductants (PDF, 27 KB) -
Transductants were generated as described by Ehrhardt et. al., using the BW5147 mouse T-cell line. Control and transduced cells were incubated with 12CA5 anti–HA (Roche) or with human IgA, IgM, IgG1, IgG2, IgG3, IgG4, or heat-aggregated IgG (100µg/mL; Sigma-Aldrich, St. Louis, MO) before staining with PE-conjugated goat anti–human Ig and analysis by cell surface immunofluorescence. Control staining is shown in grey. Ehrhardt GRA, Davis RS, Hsu JT, Leu C-M, Ehrhardt A, and Cooper MD. The inhibitory potential of Fc receptor homolog 4 on memory B cells. Proc Natl Acad Sci U S A. 2003;100:13489-13494.
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