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Blood, Vol. 105, Issue 11, 4407-4415, June 1, 2005
Derivation of 2 categories of plasmacytoid dendritic cells in murine bone marrow
Blood Pelayo et al.
105: 4407
Supplemental materials for: Pelayo et al, Vol 105, Issue 11, 4407-4415
Files in this Data Supplement:
- Figure S1. Ly6C- cohort expresses NK lineage markers. (JPG, 30 KB)
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B220+CD19- CD11cLoLy6C- cohort from C567BL/6 marrow was stained with DX-5 or NK1.1 antibodies. Dotted lines: B220+CD19- CD11cLoLy6C+ pDC.
- Figure S2. Neither subset of pDC can differentiate into myeloid, erythroid, B, NK or T lineage cells. (JPG, 64KB)
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The GFP+ and GFP- (A) pDC subsets described in Figure 6 were sorted, and 1,000 cells were cultured in Iscove's MDM-based methylcellulose for 8 days. Colonies were then classified as BFU-E, CFU-GM and CFU-GEMM. (B) pDC subsets were sorted and 5,000 cells were cultured in stromal cell- and FCS-free culture medium with growth factors. Cells were harvested and stained with antibodies to CD19 and DX-5. BM Lin-c-Kit+ cells were cultured as positive control. (C) pDC subsets were sorted and cultured for two weeks with SCF and IL-7 in a fetal thymus organ culture system. Embryonic day 12 fetal thymus was used as a positive control.
- Figure S3. RAG1 expressing pDC is a stable population in peripheral lymphoid tissues. (JPG, 44KB)
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pDC from spleen and BM of homozygous RAG1/GFP knock-in mice were resolved into GFP+ and GFP- populations (top histograms). Dotted lines: pDC from WT C57BL/6 mice. cDNA samples from sorted pDC1 and pDC2 of heterozygous mice were evaluated for RAG1 expression by RT-PCR (bottom panel) and compared to BM pro-B cells.
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