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Blood, Vol. 105, Issue 6, 2372-2379, March 15, 2005
Roles of vascular endothelial growth factor receptor 3 signaling in differentiation of mouse embryonic stem cell–derived vascular progenitor cells into endothelial cells
Blood Suzuki et al.
105: 2372
Supplemental materials for: Suzuki et al, Vol 105, Issue 6, 2372-2379
Files in this Data Supplement:
- Figure S1. A low dose of sVEGF-C(WT) induced the endothelial differentiation of VEGFR3-transfected, but not mock-transfected, VEGFR2+ cells (JPG, 123 KB)
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VEGFR2+ cells transfected with mock and VEGFR3 (long and short forms) cDNAs were cultured in the presence of sVEGF-C(WT), which was diluted 10-fold with the supernatant of mock-transfected HEK293 cells. After 2 days, cells were doubly stained with anti–PECAM-1 (purple) and anti-SMA (brown) antibodies. Numbers of ECs and total cells were counted and shown as ECs/total (%). Scale bar, 100 µm. VEGFR2+ cells transfected with mock and VEGFR3 (long and short forms) cDNAs were cultured in the presence of sVEGF-C(WT), which was diluted 10-fold with the supernatant of mock-transfected HEK293 cells. After 2 days, cells were doubly stained with anti–PECAM-1 (purple) and anti-SMA (brown) antibodies. Numbers of ECs and total cells were counted and shown as ECs/total (%). Scale bar, 100 µm.
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