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Blood, Vol. 105, Issue 3, 1179-1186, February 1, 2005
Differential regulation of virus-specific T-cell effector functions following activation by peptide or innate cytokines
Blood Beadling and Slifka
105: 1179
Supplemental materials for: Beadling and Slifka, Vol 105, Issue 3, 1179-1186
Files in this Data Supplement:
- Figure S1. Differential regulation of cytokine production levels (PDF, 99 KB) -
Spleen cells isolated from mice at 8 days (Day 8) or 145 days (Immune) after LCMV infection were activated directly ex vivo with either peptide alone or the combination of peptide plus IL-12 and IL-18. BFA was added for the final hour of stimulation, and cytokine production by CD8+ T cells was detected by intracellular staining. Each cytokine was analyzed at the peak time of production. (A) IFN
 production after 8 hours of stimulation. (B) TNF  after 2 hours of activation. (C) CD40L after 3 hours of activation. (D) IL-2 after 3 hours of stimulation. Graphs depict MFI values for each cytokine and are the mean ± SD of 4 mice in each group. Statistical comparisons were made using a paired Student t test.
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