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Blood, Vol. 105, Issue 11, 4416-4423, June 1, 2005 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef A subset of natural killer cells achieves self-tolerance without expressing inhibitory receptors specific for self-MHC molecules Blood Fernandez et al. 105: 4416 Supplemental materials for: Fernandez et al, Vol 105, Issue 11, 4416-4423 Files in this Data Supplement: Figure S1. CI/NKG2- NK cells respond poorly when stimulated with Kb–/–Db–/– lymphoblasts (JPG, 46 KB) - Splenocytes from poly(I:C)-treated 2m–/– or B6 mice were stimulated for 5 hours in the presence of brefeldin A with Con A–induced lymphoblasts from the designated mice before staining the cells with mAbs specific for cell surface markers and intracellular IFN-. Results are depicted as the percentage ± SEM (n=3 mice) of IFN-+ cells in each subset (CI/NKG2+ or CI/NKG2–). Neither 2m–/– nor B6 CI/NKG2– NK cells responded significantly to Kb–/–Db–/– Con A blasts. Figure S2. Bone marrow rejection assay with limiting doses of bone marrow (JPG, 53 KB) - Recipient mice (2m–/– or B6) were treated with mAbs to deplete the indicated NK cell subset, followed by lethal irradiation. At day 0, a mixture of 5 × 106 each (left panel) or 2.5 × 106 each (right panel) CFSE-labeled bone marrow cells from 2m–/– Ly5.1+ and B6 (Ly5.2) mice was injected intravenously. After 3 days, splenocytes were stained with PE-conjugated anti-Ly5.1 Ab. The results are represented as graft acceptance, calculated as the ratio of 2m–/– (Ly5.1+) bone marrow cells to B6 (Ly5.1–) bone marrow cells among gated CFSE-positive cells. The data represent means ± standard errors of the means of the ratios (n = 3). The B6 cells serve as a nonrejected internal reference population. The graft acceptance ratios in control groups vary from experiment to experiment, probably due to counting errors. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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