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Blood, Vol. 106, Issue 2, 577-583, July 15, 2005
Arf6: a new player in Fc RIIIA lymphocyte-mediated cytotoxicity
Blood Galandrini et al.
106: 577
Supplemental materials for: Galandrini et al, Vol 106, Issue 2, 577-583
Files in this Data Supplement:
- Figure S1. Visualization of perforin-stained granules in NK cells by fluorescence microscopy (JPG, 24 KB)
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NK cells were stimulated with anti-CD16 mAb, incubated with P815 target cells, and stained with FITC-conjugated perforin, as described in “Materials and methods.” As shown in figure 2C, no significant difference between uninfected or recombinant vaccinia encoding Arf6 WT or Q67L-infected NK cells was observed. For simplicity, a representative example of NK/P815 conjugates (top) and isolated NK cells (bottom) is shown here.
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