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Blood, Vol. 105, Issue 11, 4247-4254, June 1, 2005
An inducible caspase 9 safety switch for T-cell therapy
Blood Straathof et al.
105: 4247
Supplemental materials for: Straathof et al, Vol 105, Issue 11, 4247-4254
Files in this Data Supplement:
- Figure S1. Correlation between transgene expression level and function of iCasp9M (PDF, 236 KB) -
(A) To determine the correlation between transgene expression and function of iCasp9M iCasp9MM IRES.GFP-transduced EBV-CTL were selected for low (mean 21), intermediate (mean 80) and high (mean 189) GFP expression. (B) Selected T-cells were incubated overnight with 10 nM CID and subsequently stained with Annexin V and 7-AAD. Indicated are the percentages of Annexin V+/7-AAD- and Annexin V+/7-AAD+ T-cells. (C) Selected T-cells were mixed 1:1 with non-transduced T-cells and incubated with 10 nM CID following antigenic stimulation. Indicated is the percentage of residual GFP-positive T-cells on day 7.
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