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Blood, Vol. 106, Issue 10, 3646-3649, November 15, 2005
Donor origin of multipotent adult progenitor cells in radiation chimeras
Blood Reyes et al.
106: 3646
Supplemental materials for: Reyes et al, Vol 106, Issue 10, 3646-3649
Files in this Data Supplement:
- Figure S1. Donor-derived BM MAPCs are not the product of cell fusion (JPG, 26.7 KB)
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Mononuclear BM cells, isolated 1 month after sex-mismatched transplantation of female GFP-positive donor BM cells into a male host, were cultured in MAPC media. MAPC colonies from passage 3 were sorted as GFP-positive or GFP-negative. FISH analysis was used to detect the Y-chromosome with an FITC-conjugated probe (A, C, and E); a Dapi counterstain (B, D, and F) was used to visualize all genetic material. (A-B) All GFP-negative host cells contained the Y-chromosome. (C-F) None of the GFP-positive donor cells contained Y-chromosomes. Arrow indicates chromosomal spread 40, XY. Original magnification, × 100 for panels A- D; × 60 for panels E and F.
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