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Blood, Vol. 106, Issue 8, 2680-2687, October 15, 2005
Fli1, Elf1, and Ets1 regulate the proximal promoter of the LMO2 gene in endothelial cells
Blood Landry et al.
106: 2680
Supplemental materials for: Landry et al, Volume 106, Issue 8, 2680-2687
Sequences and annotation files for LMO2, used to generate the alignments shown in Figure 1 of the main article.
Files in this Data Supplement:
- Human Sequence (PDF, 534 KB)
- Mouse Sequence (PDF, 415KB)
- Rat Sequence (PDF, 359 KB)
- Dog Sequence (PDF, 596 KB)
- Xenopus tropicalis Sequence (PDF, 116 KB)
- Zebrafish Sequence (PDF, 155 KB)
- Fugu Sequence (PDF, 70.1 KB)
- LAGAN Multiple Alignment (PDF, 741 KB)
- Figure S1: Determination of the amplification efficiency of distal and total LMO2 primers on a common template (PDF, 59.2 KB) -
The human LMO2 common target (B) was amplified from human bone marrow cDNA, and the mouse common target (A) was generated from 416B cells. Serial dilutions of the LMO2 common targets (which contain the annealing sites for both the distal and total LMO2 primer pairs) were used to determine the primer efficiencies by comparing respective Ct values. The two mouse primer pairs showed virtually identical amplification efficiencies (
 Ct of 0.2, as shown on the left panel, and a slope < 0.1 in the plot of log input versus delta Ct, as shown on the right). The primer pair for the total human LMO2 message was more efficient than the distal primer pair (Ct of 1.5, as shown on the left panel). To account for the differences in primer efficiencies observed, the equations of the generated relative standard curves were used to adjust threshold cycle values for total and distal LMO2 amplicons in each tissue or cell line.
- Human Annotation (XLS, 69.5 KB)
- Mouse Annotation (XLS, KB)
- Rat Annotation (XLS, 58.5 KB)
- Dog Annotation (XLS, 85.5 KB)
- Xenopus tropicalis Annotation (XLS, 16.5 KB)
- Zebrafish Annotation (XLS, 28.5 KB)
- Fugu Annotation (XLS, 19 KB)
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