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Blood, Vol. 106, Issue 13, 4210-4216, December 15, 2005
MALT1 contains nuclear export signals and regulates cytoplasmic localization of BCL10
Blood Nakagawa et al.
106: 4210
Supplemental Figure for: Nakagawa et al
Files in this Data Supplement:
- Figure S1. Subcellular localization of GFP fusion proteins containing the NES1 regulatory region (PDF, 54.1 KB) -
(A) Protein structures of various GFP fusion MALT1 deletion mutants, GFP and a full-length MALT1 expression vector. NES1 indicates the NES1 region and NES1R the NES1 regulatory region. (B) Immunofluorescence images of various GFP mutants. GFP fused to the region containing the NES1 and the NES1R (329-420AA; GFP-NES1+NES1R) was localized in both nuclei and cytoplasm, but GFP-NES1 in cytoplasm only (Figure 3B), indicating that NES1R modified the cytoplasmic localization of GFP-NES1. However, GFP fused to the NES1R alone (381-420AA; GFP-NES1R) was not able to change subcellular localization of GFP, indicating that NES1R is not actively involved in nuclear localization.
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