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Blood, Vol. 106, Issue 1, 287-295, July 1, 2005
NUP98-HOXD13 transgenic mice develop a highly penetrant, severe myelodysplastic syndrome that progresses to acute leukemia
Blood Lin et al.
106: 287
Supplemental Table and Figures for: Lin et al, Vol 106, Issue 1, 287-295
Files in this Data Supplement:
- Table S1. Serial CBC results from mice that developed acute leukemia (PDF, 131 KB)
- Figure S1. Concurrent erythroid leukemia and pre-T LBL in mouse no. 1018 (PDF, 278 KB) -
(A,B) Erythroblasts in peripheral blood (A) and bone marrow (B), stained with MGG. (C,D) Erythroblasts invading the liver, stained with H&E (C) and anti-CD3 (D). (E,F) T-lymphoblasts invading the lung, stained with H&E (E) or anti-CD3 (F); inset original magnification, × 400. (G) Genomic DNA from non-transgenic control liver (C), or from diseased mouse thymus (Th), liver (Li), kidney (Ki), or spleen (Sp), digested with SsfI and hybridized to a TCRB probe. An identical clonal rearrangement is evident in the thymus and lung lanes. CD3 staining is indicated below. ND indicates not done.
- Figure S2. NHD13 mice generated on a C57Bl6 background (PDF, 158 KB) -
(A) Top panel: Northern blot hybridization of RNA from G2 mice (C57Bl6) and C1 mice (FVB/N) to an NUP98 cDNA probe. Tissues are as indicated in the legend to Figure S1. Bottom panel: ethidium-stained gel prior to transfer. (B) Survival curve of C57Bl6 transgenic mice. One non-transgenic control mouse was found dead of an unknown cause, without evidence of hematopoietic malignancy.
- Figure S3. Myeloid leukemia with maturation in NHD1 transgenic mouse no. 2554 (PDF, 121 KB) -
(A) Massive hepatosplenomegaly. (B) Bone marrow myeloblasts and immature forms, including large-ring neutrophils, stained with MGG. (C,D) Perivascular invasion of liver, stained with H&E (C) or anti-MPO (D). (E) FACS analysis on bone marrow stained with Mac1 and Gr1.
- Figure S4. NHD13 transgene expression in diseased mice (PDF, 68.1 KB) -
RT-PCR showing expression of NHD13 in various leukemic samples from transgenic FVB and C57Bl6 mice. Mouse no. 1018 developed two simultaneous leukemias (see “NHD13 mice develop a wide spectrum of hematologic malignancies”); the pre-T LBL is represented by thymus tissue, and the erythroleukemia is represented by liver tissue.
- Figure S5. Immortal cell lines established from NHD13 leukemic cells (PDF, 282 KB) -
(A) MGG-stained cytospin of a cell line established from mouse no. 254. (B,C) FACS analysis of the mouse no. 254 cell line stained with Mac1 and Gr1 or Sca-1 and c-kit.
- Figure S6. Meis1 expression is not up-regulated in NHD13 leukemia (PDF, 367 KB) -
Top panel: Meis1 RT-PCR of bone marrow cDNA from NHD13 mice with megakaryocytic leukemia (mouse no. 1017), undifferentiated leukemia (mice nos. 1019 and 2413), pre-T LBL (mouse no. 1901), and myeloid leukemia (mouse no. 254). Bottom panel: β-actin RT-PCR. Numbers above the panel indicate dilution of templates (1 = 1/10, 2= 1/100, 3=1/1000).
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