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Blood, Vol. 106, Issue 2, 584-592, July 15, 2005 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef ICAM-1 regulates neutrophil adhesion and transcellular migration of TNF--activated vascular endothelium under flow Blood Yang et al. 106: 584 Supplemental materials for: Yang et al, Vol 106, Issue 2, 584-592 Files in this Data Supplement: Video S1. An adherent PMN (identified by yellow arrow) transmigrates through HUVECs activated for 24 hours with TNF-α at a nonjunctional site (MOV, 97 KB) - Supplement to Figure 1. The endothelial cell junctions are labeled in red using Alexa568tagged VE-cadherin mAb Tea1/31. The DIC and fluorescence images were captured every 15 seconds and overlaid using MetaMorph and ImageJ software. Time is in seconds. Bar, 10 µm. Video S2. ICAM-1 clusters around two PMNs undergoing TEM at nonjunctional sites, as assessed by live-cell digital imaging (MOV, 278 KB) - Supplement to Figure 3. ICAM-1GFP iHUVECs were stimulated with TNF-α for 4 hours and are labeled in red with Alexa568-tagged VE-cad mAb. PMNs were drawn across the monolayer at 1 dyne/cm2, and adhesion and TEM were monitored as described in “Materials and methods.” ICAM-1 (green) clusters around 2 PMNs that transmigrate at different nonjunctional sites (arrows). Bar, 10 µm. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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