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Blood, Vol. 106, Issue 4, 1175-1182, August 15, 2005
Plasma chemokine levels correlate with the outcome of antiviral therapy in patients with hepatitis C
Blood Butera et al.
106: 1175
Supplemental materials for: Butera et al, Vol 106, Issue 4, 1175-1182
Files in this Data Supplement:
- Figure S1. CXCR3 expression is unchanged by cryopreservation (JPG, 66 KB)
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(A) HCV patient PBMCs, prepared as described in “Patients, materials, and methods,” were stained for flow cytometry either immediately or after cryopreservation and thawing. Staining with anti-CD20, anti-CD3, and anti-CXCR3 was evaluated by flow cytometry. B lymphocytes, identified by forward and right-angle light scatter and by CD20+CD3– staining patterns, were analyzed for expression of CXCR3. The heavy line shows the CXCR3 staining pattern of B cells that had been cryopreserved; the shaded area shows the CXCR3 staining pattern of B cells that were stained immediately after isolation. At least 2000 B cells were analyzed for each plot. (B) PBMCs from healthy controls were thawed and stimulated for 3 days with 2 µg/mL phytohemaglutinin (Sigma, Saint Louis, MO). After stimulation, cells either were cryopreserved and thawed, then stained (heavy line), or were stained immediately (shaded area) as for panel A. Unstimulated cells are shown for comparison (thin line). CXCR3 staining of gated B cells is shown. At least 2000 B cells were analyzed for each plot.
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