Document S1. Supplemental information on methods and results (PDF, 759 KB) -
This document contains details on sample preparation, quality control, and expression validation using quantitative real-time RT-PCR of the microarray analysis. Supplemental information on additional results is listed.
Figure A: Mercapopturine graphical pathway diagram.
Figure B: Treatment regimens and bone marrow sampling times.
Figure C: “Unsupervised” hierarchical clustering of ALL treated with MP alone and with the combination MTX+MP, and effect of low- versus high-dose MTX on the combination MTX+MP data set.
Figure D: Genes negatively correlated with GI50 values in the 60 NCI tumor cell lines and positively correlated with TGN accumulation in ALL cells after MP treatment.
Table A: Patient characteristics.
Table B: Association between demographic characteristics and TGN concentration in patients treated with MP and with the combination MTX+MP.
Table C: Association between TGN concentration and plasma MP and MP-metabolite concentrations in patients treated with MP and with the combination MTX+MP.
Table D: Genes associated with TGN concentration in ALL cells of patients treated with MP.
Table E: Genes associated with TGN concentration in ALL cells of patients treated with the combination MTX+MP.
