|
|
Blood, Vol. 107, Issue 2, 806-812, January 15, 2006
How to drain without lymphatics? Dendritic cells migrate from the cerebrospinal fluid to the B-cell follicles of cervical lymph nodes
Blood Hatterer et al.
107: 806
Supplemental materials for: Hatterer et al
Files in this Data Supplement:
- Figure S1. Characterization of rat bone marrow-derived myeloid DCs (PDF, 116 KB) -
(A) Immature DCs generated from rat bone marrow cultures express OX42 (CD11b/c), OX62 and low membranous levels of CD80 as assessed by FACS analysis. In each quadrant are indicated the percentage of cells displaying fluorescence intensity above the background level obtained with a control antibody (grey curve), the mean fluorescence intentisity (MFI) and the factor of MFI increase as compared to control MFI. (B) After 24h stimulation with LPS 100 ng/ml, DCs all harbor dendrites detectable with hematein-eosin coloration (left panel) and stained using OX6 antibody directed against MHC class II molecules (right panel). (C) Allogeneic T lymphocytes were stimulated with graded concentrations of irradiated LPS-stimulated DCs. After 72 h culture, cells were pulsed with 2 µCi/well of 3H-thymidine (Amersham Biosciences) for 18h. Cells were then harvested, and incorporated thymidine was quantified in a direct beta counter. Results are presented as mean counts per minute ± SD of triplicate cultures. c.p.m: counts per minute. Scale bars: 2 µm (B, left and right panels). Data shown are representative of at least 3 experiments.
|
|
