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Blood, Vol. 107, Issue 1, 184-186, January 1, 2006
Use of interleukin 7 receptor- knockout donor cells demonstrates the lymphoid independence of dendritic cells
Blood Takeuchi and Katz
107: 184
Supplemental materials for: Takeuchi and Katz
Files in this Data Supplement:
- Figure S1. Cellular chimerism in reconstituted recipients (JPG, 188 KB)
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The results are expressed as mean % chimerism ± SD, followed by mean % population ± SD in each lymphoid organ (values within parenthesis). All the myeloid cells (PMN/monocytes, M ) were well reconstituted, while lymphoid lineage cells (except for NK cells) were less well reconstituted by IL-7R KO donor cells as compared to those by WT donor cells. PMN: polymorphonuclear neutrophils, M: macrophage, DN: CD4 and CD8 double negative, DP: CD4 and CD8 double positive, SP: single positive. NK: natural killer. *CD11b+ DC is also inevitably included in this category (CD11b+Gr-1–). (N=4 each)
- Figure S2. Definition of splenic- and pLN-DC subsets and BM-plasmacytoid DC (JPG, 134 KB)
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(A-C) DC subsets (CD11b+-, CD8+- and plasmacytoid DC) are defined using CD11b-, CD8- and B220 mAbs along with CD11c expression in (A) recipient spleen, (B) pLN and (C) BM that were examined for chimerism at 4 weeks after reconstitution.
- Figure S3. Examination of lymphoid (or IL-7Rα) dependency of thymic DC subsets and plasmacytoid DCs in spleen, thymus, pLN and BM. (DC subsets were defined using CD8α-, CD11b- and PDCA-1 mAbs along with CD11c expression (JPG, 113 KB)
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(A) Thymic CD11b+-, CD8+-DC and plasmacytoid DCs in spleen, pLN, thymus, and BM. (B-D) There was no significant difference in the chimerism of (B) thymic CD11b+- and (C) CD8+-DC subsets or (D) plasmacytoid DCs in recipient spleen, thymus, pLN and BM when WT- and IL-7R KO-reconstituted mice were compared at 4 weeks after reconstitution. pDC; plasmacytoid DC. (N=5 each)
- Figure S4. TNCB-painting enhance LC engraftment and IL-7Rα KO donor cells reconstitute LC but not epidermal αβT cells (JPG, 107 KB)
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Donor-derived LC (MHC II+ and CD45.2+) were present in recipient epidermis (arrow), especially in irritated (TNCB-painted) ears. Note that WT donor cells also reconstitute MHC II– populations (arrow with break line) in the epidermis, while such populations were rarely observed in IL-7R KO-reconstituted mice. Such engrafted donor-derived MHC II- cells that were reconstituted by WT donor cells were mostly CD3 + and TCR  + T cells (data not shown), but not   T cells.
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