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Blood, Vol. 107, Issue 7, 2662-2672, April 1, 2006 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Core erythropoietin receptor signals for late erythroblast development Blood Menon et al. 107: 2662 Supplemental materials for: Menon et al Files in this Data Supplement: Figure S1. Epo activation of Stat1 in wild-type-EpoR erythroblasts (JPG, 45.5 KB) - Erythroblasts were expanded from wt-EpoR, EpoR-HM and EpoR-H bone marrow preparations. Isolated CD71highTer119neg cells then were washed, incubated for 6 hours in the absence of hematopoietic cytokines, and stimulated with Epo for 10 minutes. Lysates then were prepared directly, and were analyzed via western blotting for levels of activated PY-Stat1 and total Stat1. In wt-EpoR erythroblasts, Epo-activation of Stat1 was detected, but only upon prolonged ECL exposure. Figure S2. Epo induction of Cis-1 transcript expression in wt-EpoR and EpoR-H, but not EpoR-HM erythroblasts (JPG, 67.1 KB) - Erythroblasts were prepared from wild-type EpoR, EpoR-H, and EpoR-HM bone marrow. Expanded cells were washed, incubated for 6 hours in the absence of hematopoietic cytokines and stimulated with Epo for the indicated intervals. Cells then were lysed in Trizol reagent and total RNA was isolated. Cis-1 transcript levels were analyzed by quantitative RT-PCR. Figure S3. p38 MAPK activation via minimal Epo receptor alleles (JPG, 68.2 KB) - wt-Epo-R, EpoR-HM, and EpoR-H erythroblasts were expanded for 3 days. CD71highTer119neg cells were then isolated, washed, and incubated for 6 hours in the absence of hematopoietic cytokines. At the subsequent indicated intervals of Epo-exposure, lysates were prepared and analyzed by western blotting for levels of activated and total p38-MAPK. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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