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Blood, Vol. 106, Issue 9, 3227-3233, November 1, 2005
Jak3 negatively regulates dendritic-cell cytokine production and survival
Blood Yamaoka et al.
106: 3227
Supplemental materials for: Yamaoka et al, Vol 106, Issue 9, 3227-3233
Files in this Data Supplement:
- Figure S1. Decreased splenocytes in Jak3–/– and γc–/– mice (JPG, 47.3 KB)
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Spleens were harvested from wild-type, Jak3–/– and  c–/– mice and the total number of cells was counted after lysis of red blood cells with ACK lysing buffer. The absolute number (mean +/- SD) of splenocytes in wild-type, Jak3–/– and c–/– mice from three separate experiments are shown.
- Figure S2. Normal development of Jak3–/– bone marrow in vivo and in vitro (JPG, 169 KB)
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(A) Bone marrow cells from wild-type (left panel) and Jak3–/– (right panel) mice were cultured for 10 days with Flt3L (100ng/mL). The resultant cells were stained with anti-CD11c antibody. Shaded histograms represent samples stained with an isotype control. The data are representative of two separate experiments. (B,C) Bone marrow cells from wild-type (CD45.1+) and Jak3–/– (CD45.2+) mice were mixed in a 1:1 ratio and injected intravenously into lethally irradiated Rag2–/– C57BL/6 congenic mice (CD45.1+). Six to eight weeks later, low-density splenic cells were stained and gated on CD11c+ (B)or CD11b+ (C) cells to analyze the repopulation with each source.
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