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Blood, Vol. 106, Issue 5, 1843-1850, September 1, 2005
Neutrophils rapidly migrate via lymphatics after Mycobacterium bovis BCG intradermal vaccination and shuttle live bacilli to the draining lymph nodes
Blood Abadie et al.
106: 1843
Supplemental Figures for: Abadie et al, Vol 106, Issue 5, 1843-1850
Files in this Data Supplement:
- Figure S1. BCG is not associated with Langerhans cells in the dermis (JPG, 93 KB)
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(A) At 12 and 72 hours after BCG-egfp injection, skin explant cells were double-stained with CD11c and anti-CD207 (Langerin) and analyzed by flow cytometry. Among the CD11c+ gated cells, only 4% at 12 hours and less than 1% at 72 hours after injection were CD207+ Langerhans cells. (B) Ear cryosections from BCG-egfp and mock-injected mice were immunostained with anti-CD207. Co-localization of BCG (green) with Langerhans cells (red) could not be observed (magnification, ×200).
- Figure S2. Langerhans cells do not carry BCG into the ADLN after vaccination in the ear dermis (JPG, 111 KB)
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(A) At 12 and 72 hours after BCG-egfp injection, ADLN CD11c+ cells were magnetically purified and stained with anti-CD207 before flow cytometry. Less than 1% of Langerhans cells (CD11c+, CD207+) were EGFP+, ie, BCG-infected. (B) ADLN sections from BCG-egfp or mock-injected mice were immunostained with anti-CD207. At 12 and 72 hours after injection, LCs (red) were detected in the paracortex of the ADLN but did not colocalize with BCG (green) that remained in the subcapsular space (magnification, ×100).
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