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Blood, Vol. 107, Issue 2, 454-462, January 15, 2006
Role of dendritic cell–derived CXCL13 in the pathogenesis of Bartonella henselae B-rich granuloma
Blood Vermi et al.
107: 454
Supplemental materials for: Vermi et al
Files in this Data Supplement:
- Figure S1. Immunohistochemical detection of T-bet in monocytoid B-cells (JPG, 177 KB)
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To evaluate the specificity of T-bet expression in monocytoid B-cells serial sections from a reactive lymph node were stained for T-bet, CD20, CD3 and with an isotype matched irrelevant antibody (a-d). The aggregates of T-bet+cells correspond to CD20 extrafollicular B lymphocytes (*); note that the CD3+ T-cell areas are located outsite the T-bet+ clusters. No reactivity is found using the isotype-matched antibody as negative control.
Similar findings are noted in the CSD granuloma: the T-bet+ population within the granuloma largely correspond to the distribution of the CD20+ B lymphocytes typically found in this lesion; note that CD3+T cells are mainly concentrated at the periphery of the granuloma; no staining is obtained with the isotype matched antibody.
[Panel (a) and (b) correspond to Figure 6e and 6f of the manuscript, respectively; panel (e) to Figure 6g].
- Figure S2. Immunohistochemical detection of CXCL8 protein in CSD granuloma (JPG, 86.2 KB)
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CSD granuloma: serial cryostat sections stained with anti-CXCL8 and with an isotype-matched irrelevant antibody as negative control (b). The strong reactivity shown in (a) is completely abolished in (b).
[Panel (a) corresponds to Figure 7f of the manuscript].
- Figure S3. Immunohistochemical detection of IL-10 protein in CSD granuloma (JPG, 138 KB)
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CSD granuloma: serial cryostat sections stained with anti-IL-10 (a) and with an isotype-matched irrelevant antibody as negative control, showing that the strong reactivity in (a) is completely absent in (b). Similar negative stain was obtained with the omission of the primary antibody. In (c) a reactive lymph node was also stained with anti-IL10, showing that no positive cells are identified.
[Panel (a) corresponds to Figure 7e of the manuscript].
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