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Blood, Vol. 106, Issue 10, 3432-3439, November 15, 2005
T-bet is required for optimal proinflammatory CD4+ T-cell trafficking
Blood Lord et al.
106: 3432
Supplemental materials for: Lord et al, Vol 106, Issue 10, 3432-3439
Files in this Data Supplement:
- Table S1. Surface expression (%) and mean fluorescence intensity (MFI) on DO11.10 (WT) and DO11.10 × T-bet–/– transgenic (KO) primary CD4+ T cells activated under different polarizing conditions (JPG, 40 KB)
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Boxed results indicate significant staining over isotype control.
- Table S2. CCR5 surface expression (%) and mean fluorescence intensity (MFI) on different genotypes of primary CD4+ cells activated under Th1 or Th2 polarizing conditions (JPG, 27 KB)
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- Figure S1. In vivo trafficking of adoptively transferred WT and T-bet–/– antigen-specific T cells activated under Th2 polarising conditions (JPG, 66 KB)
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(A,B) Flow cytometric analysis of DO11.10 and DO11.10 × T-bet–/– CD4+ T cells. Percentages of cells positive for CD4 and the clonotypic antibody KJ1-26 in various secondary lymphoid organs and inflamed peritoneum are indicated. ILN indicates inguinal lymph nodes; MLN, mesenteric lymph nodes; and PL, peritoneal lavage.
- Figure S2. Real-time PCR analysis of FucTVII mRNA levels in WT and T-bet CD2-Tg primary CD4+ cells activated under different polarizing conditions (normalized to β-actin) (JPG, 40 KB)
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- Figure S3. Real-time PCR analysis of TPST-1 (A) and TPST-2 (B) mRNA levels in WT and T-bet CD2-Tg primary CD4+ cells activated under different polarizing conditions (normalized to β-actin) (JPG, 33 KB)
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- Figure S4. Real-time PCR analysis of CCR5 mRNA levels in WT and T-bet CD2-Tg primary CD4+ cells activated under different polarizing conditions (JPG, 41 KB)
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Data are mean plus or minus SEM.
- Figure S5. Transwell migration of WT and T-bet CD2-Tg primary CD4+ cells in response to recombinant CCL4 (1nM) (JPG, 35 KB)
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