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Blood, Vol. 107, Issue 2, 423-430, January 15, 2006
C1qTNF–related protein-1 (CTRP-1): a vascular wall protein that inhibits collagen-induced platelet aggregation by blocking VWF binding to collagen
Blood Lasser et al.
107: 423
Supplemental materials for: Lasser et al
Video. Images were acquired as described in the methods section of the main article. Platelets were labeled by incubating the blood sample with 0.2M mepacrine for 10 min in the dark and the blood was perfused over the slide containing the immobilized collagen or vWF.
CTRP-1 blocks adhesion of platelets on a collagen type I-coated surface at high shear. Citrated whole human blood was incubated with either vehicle alone (Video 1) or with 20 µg/mL CTRP-1 (Video 2) and was perfused over collagen type I coated coverslips through a flow chamber at 50 dynes/cm2. Whole human blood flowing over a collagen-coated surface in the absence of CTRP-1 rapidly leads to the formation of platelet aggregates visualized in the movie clip (Video 1). The formation of these platelet aggregates and also of single platelets sticking and rolling along the collagen surface is prevented in the presence of CTRP-1 (Video 2).
CTRP-1 does not affect “rolling” of platelets on a vWF-coated surface. Citrated whole human blood was incubated with either vehicle alone (Video 3) or with 20 µg/mL CTRP-1 (Video 4) and was perfused over vWF-coated coverslips through the flow chamber at 50 dynes/cm2. Whole human blood flowing over a vWF-coated surface (Video 3) in the absence of CTRP-1 leads to the formation of smaller static platelet aggregates (thrombi) as compared to the collagen-coated surface. In addition numerous single platelets “rolling” over the surface at a slow rate can be seen in the movie clip. A very similar picture is seen in the presence of CTRP-1 (Video 4), suggesting CTRP-1 has no significant inhibitory effect on the interaction of platelets with vWF. The movie clip might suggest a small difference though, as the size of thrombis appears to be smaller when compared to the thrombi forming in the absence of CTRP-1.
Files in this Data Supplement:
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2I domain. Each bar represents the mean ± SD of 6 separate measurements. Differences were significant comparing vehicle control and