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Blood, Vol. 107, Issue 4, 1468-1475, February 15, 2006
Regulation of ITAM-positive receptors: role of IL-12 and IL-18
Blood Ortaldo et al.
107: 1468
Supplemental material for: Ortaldo et al
Files in this Data Supplement:
- Figure S1. Evaluation of short versus long NKG2D mRNA (JPG, 140 KB)
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PCR utilizing oligonucleotides specific for short versus long NKG2D, was performed on RNA isolated from untreated and IL-2 rebound liver and spleen lymphocytes. The relative expression of the long (panel A) and short (panel B) form of NKG2D are shown normalized to ribosomal RNA. Panel C contrasts the relative increase induced by IL-2 treatment in vivo relative to the control spleen or liver for the long (black bars) or short (striped bars) forms of NKG2D. Panel D express the data as a ratio of long to short indicating the relative expression levels in each tissue before and after IL-2 treatment.
- Figure S2. Evaluation of nuclear versus cytoplasmic mRNA for IFN-γ (JPG, 29.5 KB)
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NK cells were lysed and fractionated as described in materials and methods. RPA was performed on nuclear and cytoplasmic RNAs using [33P]UTP labeled exon 1-intron 1 (E1-I1), exon 3-intron 3 (E3-I3), and L32 rRNA riboprobes. The exon-intron probes hybridize and protect unspliced nuclear IFN- pre-mRNA whereas the exon portion of the exon-intron probes recognize only the spliced form of the IFN- mRNA in both the nucleus and cytoplasm. L32 was used as a control for RNA input between samples.
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