|
|
Blood, Vol. 106, Issue 13, 4131-4138, December 15, 2005
BH3-only proteins Puma and Bim are rate-limiting for  -radiation– and glucocorticoid-induced apoptosis of lymphoid cells in vivo
Blood Erlacher et al.
106: 4131
Supplemental materials for: Erlacher et al
Files in this Data Supplement:
- Figure S1: Impact of whole body γ-radiation on lymphocytes from peripheral blood (PDF, 82.6 KB) -
Animals of the indicated genotypes were exposed to the indicated doses of
 -radiation or left untreated. Blood samples were collected 20 h after treatment, white blood cell counts determined in a hemocytometer and samples stained with fluorescence-conjugated antibodies specific for CD4 and CD8 or IgM and IgD to identify mature T and B cells, respectively by flow cytometry. The total cellularity of T cells and B cells in peripheral blood is depicted. Bars represent means ± SE of 3-6 animals of each genotype and treatment regimen used in 3 or more independent experiments.
- Figure S2: γ-radiation induced apoptosis of CD4-8- double negative thymocytes depends on p53 and can be blocked by Bcl-2 overexpression (PDF, 67.1 KB) -
Animals of the indicated genotypes were exposed to the indicated doses of whole body -radiation and were sacrificed 20 h thereafter. Thymi were harvested, single cell suspensions counted, stained with fluorescence-conjugated antibodies to CD4 and CD8 and analyzed by flow cytometry. Absolute numbers of CD4-8- thymocytes are depicted. Bars represent means ± SE of 3-6 animals of each genotype and treatment regimen used in 3 or more independent experiments.
|
|
