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Blood, Vol. 107, Issue 1, 222-231, January 1, 2006
Targeting endoplasmic reticulum protein transport: a novel strategy to kill malignant B cells and overcome fludarabine resistance in CLL
Blood Carew et al.
107: 222
Supplemental materials for: Carew et al
Files in this Data Supplement:
- Figure S1. Brefeldin A stimulates apoptosis in multiple myeloma cells (JPG, 90.5 KB)
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(A) U266 and NCI-H929 cells were treated with the indicated concentrations of BFA for 48h. Each bar represents the mean of 3 independent experiments, error bars indicate the SEM. (B-C) Time course of brefeldin A-induced apoptosis in U266 (B) and NCI-H929 (C) multiple myeloma cells. Cells were treated for 24 h and 48 h with the indicated concentrations of brefeldin A. Apoptosis was measured by Annexin-V-FITC/PI double-staining (shown) and active caspase-3 staining (data not shown). n =3, error bars indicate the SEM for each condition. (D) U266 and NCI-H929 myeloma cells were treated with 30 and 100 ng/mL brefeldin A for 16 h. Secreted levels of VEGF were quantitated in the culture medium by ELISA as described in the Materials and Methods. Bars represent the mean of 2 assays, error bars indicate the SEM.
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