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Blood, Vol. 107, Issue 6, 2446-2452, March 15, 2006
Delta-like1-induced Notch1 signaling regulates the human plasmacytoid dendritic cell versus T-cell lineage decision through control of GATA-3 and Spi-B
Blood Dontje et al.
107: 2446
Supplemental Table and Figure for: Dontje et al
Files in this Data Supplement:
- Table S1. DL1 inhibits the development of fetal liver CD34+CD38— progenitor cells into pDC (PDF, 21.1 KB) -
Human fetal liver was obtained from elective abortions and was contingent on informed consent. Gestational age was determined by ultrasonic measurement of the diameter of the skull and ranged from 14 to 20 wk. Fetal liver CD34+CD38— cells were isolated as described previously7. The absolute cell numbers represent numbers of pDC output cells derived from 50,000 input CD34+CD38— progenitor cells. The ratios are representative for the relative increase or decrease of pDC co-cultured with DL1 compared to the control co-cultured cells.
- Figure S1. OP9-DL1 and OP9-Jag1 support Notch dependent T cell development from thymic progenitor cells (PDF, 229 KB) -
(A) (first page) CD34+CD1a— cells were co-cultured with OP9-DL1 and OP9-Jag1 or control cell lines for 4 weeks in the presence or absence of 10 µM  -secretase inhibitor DAPT. Cells were analyzed for expression of CD4 and CD8 every week. (B) (second page) Cells were analyzed for expression of CD7 and CD1a every week. Experiment is representative of three.
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