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Blood, Vol. 107, Issue 11, 4449-4457, June 1, 2006
Uncoupling of T-cell effector functions by inhibitory killer immunoglobulin–like receptors
Blood Henel et al.
107: 4449
Supplemental materials for: Henel et al
Files in this Data Supplement:
- Figure S1. The anti-KIR2DL2 antibody CH-L does not block the inhibitory effect of KIR2DL2 (PDF, 20.3 KB) -
KIR2DL2+ T cells were coincubated with either SEB-coated 721.221 cells or with 721.221/HLA-Cw3 cells. T cells were incubated for 30 minutes at 4°C with the anti-KIR2DS2/DL2/3 mAb CH-L, washed twice with PBS, and then resuspended in complete media. 50,000 T cells were co-cultured with 25,000 SEB-coated 721.221/HLA-Cw3 or 721.221 cells for 48 hours. IFN- secreted into the supernatant was quantified by ELISA. Results are shown as mean ± SD of triplicate cultures. CH-L at the concentration used did not block KIR2DL2 function and was therefore used with the same experimental conditions in the confocal imaging experiments.
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