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Blood, Vol. 107, Issue 10, 3933-3939, May 15, 2006
HTLV-1 Tax protects against CD95-mediated apoptosis by induction of the cellular FLICE-inhibitory protein (c-FLIP)
Blood Krueger et al.
107: 3933
Supplemental materials for: Krueger et al
Files in this Data Supplement:
- Figure S1. Analysis of apoptosis by FSC/SSC yields comparable results to analysis of sub-diploid DNA content
(PDF, 65.5 KB) -
Jurkat cells were treated with the indicated concentrations of LZ-CD95L or left untreated for 16 h. Apoptosis was analyzed in parallel by FSC/SSC analysis (left panels) and according to the method of Nicoletti et al. (right panels). Numbers in FACS plots represent percentage of cells in the apoptosis gate.
- Figure S2. Tax expressing HTLV-1 infected cell lines derived from patients express Bid and elevated levels of c-FLIP
(PDF, 119 KB) -
A) HTLV-1 infected cell lines ATL-3, CHAMP and SP, which were derived from patients, were incubated for 16 h with the indicated concentrations of agonistic anti-CD95 antibody anti-APO-1. Apoptosis was determined by analyzing subdiploid-DNA by FACS. Specific apoptosis was calculated as decribed in Material and Methods. B) Expression levels of Tax in HTLV-1 infected cells. Lysates of non-infected (CEM) and HTLV-1 infected (HuT-102, ATL-3, CHAMP, and SP) cells were subjected to SDS-PAGE and Western blot analysis with antibodies against Tax. Analysis of Erk expression served as control for equal protein loading. C) Expression of c-FLIP and Bid in HTLV-1 infected cells. Lysates of CEM, HuT-102, ATL-3, CHAMP, and SP cells were subjected to SDS-PAGE and Western blot analysis with antibodies against c-FLIP and Bid. Analysis of Erk and FADD expression served as control for equal protein loading.
- Figure S3. Representation of non-normalized data from three independent siRNA experiments as described in Figure 7B
(PDF, 51.4 KB) -
Mean values are indicated by hyphens. One-tailed p-values were calculated using a paired-samples t-test.
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