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Blood, Vol. 107, Issue 6, 2544-2547, March 15, 2006
c-FLIP confers resistance to FAS-mediated apoptosis in anaplastic large-cell lymphoma
Blood Oyarzo et al.
107: 2544
Supplemental materials for: Oyarzo et al
Files in this Data Supplement:
- Figure S1. Apoptotic cell death of control Mac2a cells after treatment with CH11 (JPG, 45.6 KB)
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A. Annexin V binding assay using flow cytometry confirmed apoptotic cell death of Mac2a cells after treatment with CH-11 antibody. At 20 hours after treatment, the percentage of Annexin V-positive cells increased from 11% (control, untreated cells) to 52%. B. Apoptotic cell death of Mac2a cells after treatment with CH-11 antibody was associated with cleavage of caspase 8, downregulation of c-FLIP and upregulation of FADD compared with control (untreated) cells.
- Figure S2. Expression of extrinsic apoptotic pathway proteins in ALCL cell lines (JPG, 48.7 KB)
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Expression of components of the death-inducing signaling complex (DISC) in two ALK+ ALCL (Karpas 299 and SU-DHL-1) cell lines shown by Western blot methods. The Hodgkin lymphoma cell line, L-1236, served as positive control for expression of the DISC proteins as reported previously. 15 A CD30+ T-cell lymphoma (Mac2a), and a mantle cell lymphoma cell line (Mino) were also included in these immunoblots for comparison. 15. Mathas S, Lietz A, Anagnostopoulos I, et al. c-FLIP mediates resistance of Hodgkin/Reed-Sternberg cells to death receptor-induced apoptosis. J Exp Med. 2004;199:1041-1052.
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