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Blood, Vol. 107, Issue 5, 2153-2161, March 1, 2006
A subset of human rapidly self-renewing marrow stromal cells preferentially engraft in mice
Blood Lee et al.
107: 2153
Supplemental materials for: Lee et al
Files in this Data Supplement:
- Table S1. SNPs used as specific markers for donor cells (PDF, 12.8 KB) -
Two SNPs (SNP 1858822 and SNP 397272) in the collagen I A2 gene were selected for the identification of engraftment.
- Table S2 Mismatched SNP-allele specific primers (PDF, 20.5 KB) -
To enhance amplification of the complementary alleles, we synthesized SNP-allele specific primers containing one extra mismatch in one of the penultimate nucleotides.
- Figure S1. Sequences of SNPs region and SNPs-specific primers (PDF, 47.1 KB) -
DNA sequencing of amplified SNP region by COL1A2 primers were used to define the genotype of the selected SNP.
- Figure S2 (PDF, 30.9 KB) -
Amplification of SNPs by PCR using three reverse mismatched primers and a forward wild type primer was shown. *SNP specific amplifications without negative amplifications.
- Figure S3. Standard curves for the competitive allele-specific SNP assay (PDF, 35.8 KB) -
a: Real time PCR with the forward and the reverse primer for the A/A allele in samples that contained 200 ng of mouse DNA, 1 ng of the G/G allele and 1pg to 1 ng of the A/A allele. b: Real time PCR with the forward primer and the reverse primers for the G/G allele in samples that contained 200 ng mouse DNA, 1 ng of the A/A allele and 1pg to 1 ng of the G/G allele. c: Same sample as in Panel a amplified with the primers for the G/G allele. d: Same sample as in Panel b amplified with the primers for the A/A allele.
- Figure S4 (PDF, 23.2 KB) -
A. Engraftment in tissues after tail vein injections of a 1:1 mixture of 2.5 × 105 RS-MSCs and 2.5 × 105 SR-MSCs. Data from Fig. 3 expressed as average % of injected cells.
B. Engraftment in brain after intracranial injections of either 105 RS-MSCs from one donor or a 1:1 mixture of 5 × 104 RS-MSCs from one donor and 5 × 104 SR-MSCs from a second donor. Data are from Fig. 4 expressed and average % of injected cells.
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