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Blood, Vol. 107, Issue 1, 87-97, January 1, 2006
Early block to erythromegakaryocytic development conferred by loss of transcription factor GATA-1
Blood Stachura et al.
107: 87
Supplemental materials for: Stachura et al
Files in this Data Supplement:
- Figure S1. GATA-1 induces the expression of megakaryocyte and erythroid differentiation markers in distinct cell populations (JPG, 67.4 KB)
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A. Double staining of MIGR1 and GATA-1-transduced G1ME cells with Ter119 and GPIb antibodies. Percentages denote the fraction of GFP+ cells staining with respective markers. B. Cells were stained separately for GPIb (red) or Ter119 (green) and then analyzed according to forward and side scatter which reflect size and granularity, respectively. The GPIb+ cells are large and granular, reflecting a megakaryocytic phenotype (see reference 34), while the Ter119+ cells are grouped in a distinctly different population with lower forward and side scattering.
- Figure S2. GATA-1 induces proliferation arrest of G1ME cells (JPG, 85.2 KB)
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A. Percentage live cells were assessed using a vital dye after transduction with MIGR1 or GATA-1 virus. There is no increased death in the population of cells infected with GATA-1 virus. B. Proliferative capacity of cells infected with MIGR1 or GATA-1 retrovirus. Immediately after infection (Day 0) the cells were stained with the membrane dye PKH-26. PKH-26 fluorescence decreases logarithmically every time a cell divides as the dye is distributed into two daughter cells. Proliferative Index (PI), which reflects the increase in cell number in the culture over the course of the experiment, was calculated at Day 4 post infection by ModFit LT software. Note that the PI for cells transduced with GATA-1 is lower than cells infected with MIGR1. Non-transfected cells exhibited an identical PI to MIGR1-transduced cells (not shown).
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