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Blood, Vol. 107, Issue 10, 3868-3875, May 15, 2006 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Characterization of the megakaryocyte demarcation membrane system and its role in thrombopoiesis Blood Schulze et al. 107: 3868 Supplemental Figure and Video for: Schulze et al Files in this Data Supplement: Figure S1 (JPG, 76.1 KB) - (A) Schema of the domain structure and exon organization of the PI4PK protein and gene locus, respectively. The drawings reveal the only difference between the full-length transcript reported previously (“canonical”) and an alternative transcript we isolated from a MK cDNA library. In this transcript the usual first exon (1a) is replaced by an alternative and previously unknown exon (1b), which provides a different start codon. The resulting protein (Δ59N) lacks 59 N-terminal residues that normally form the first -helix. (B) The Δ59N variant possesses enzyme activity for PI5P phosphorylation equivalent to those of full-length PI4PK and PI4PK, as revealed in a chromatographic assay. When combined in equal ratios with PI4PK or PI4PK, Δ59N did not act as a dominant inhibitor (data not shown). Video 1 (MPG video, 953 KB) - Three-dimensional reconstruction of the cell shown in Figure 2F, with the outline of the invaginated DMS highlighted by EGFP-tagged PH(PLCd1). Serial fluorescent images were used for reconstruction with DeltaVision software. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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