|
|
Blood, Vol. 107, Issue 6, 2570-2577, March 15, 2006
Interferon- -stimulated marrow stromal cells: a new type of nonhematopoietic antigen-presenting cell
Blood Stagg et al.
107: 2570
Supplemental materials for: Stagg et al
Files in this Data Supplement:
- Figure S1A. Flow cytometry analysis of MSCs populations (JPG, 372 KB)
-
Primary MSCs were isolated from the femurs and tibias of C57BL/6 female mice and culture expanded in DMEM 10% FBS. Polyclonal MSCs (A), MSCs clone 4 (B), clone 6 (C) and clone 10 (D)were analyzed by flow cytometry for CD45, CD105, MHC class I (H2-Kb), MHC class II (I-Ab), CD80, CD86, CD40 and CD54 cell surface expression. Where indicated, MSCs were first treated with recombinant mouse IFN (50ng/ml) for 20hrs prior to flow cytometry analysis. Plots show isotype control IgG staining profile (doted line) versus specific Ab staining profile (thick line).
- Figure S1B (JPG, 210 KB)
-
- Figure S1C (JPG, 393 KB)
-
- Figure S1D (JPG, 203 KB)
-
- Figure S2: Soluble ovalbumin antigen cross-presentation (JPG, 121 KB)
-
C57BL/6 MSCs, DC2.4 or MEF (5 × 104 cells) were cocultured for 20hrs with ovalbumin-specific MHC class I-restricted T-T hybridomas (RF33.70; 105 cells) in the presence of increasing doses of soluble ovalbumin. Where indicated, recombinant IFN (50ng/ml final) was added to the cocultures. After 20hrs, supernatant was collected and tested for IL-2 release by ELISA (Means of triplicates ± standard deviations are shown).
|
|
