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Blood, Vol. 107, Issue 6, 2540-2543, March 15, 2006
Notch1 mutations are important for leukemic transformation in murine models of precursor-T leukemia/lymphoma
Blood Lin et al.
107: 2540
Supplemental materials for: Lin et al
Files in this Data Supplement:
- Figure S1. Effect of γ-secretase inhibition on Notch1 mutant cell lines (PDF, 48.3 KB) -
(A) F4-6 (Notch1 wild type) and 3781 (SCL/LMO1 cell line with a Notch1 PEST domain mutation) were treated with 5 µM Z-IL-CHO (GSI) or mock treated with DMSO for 72 hours. There was no detectable Notch1 protein in F4-6. Expression of ICN1 was reduced following GSI treatment of cell line 3781, which was sensitive to GSI. (* p < 0.01) (B) SCL/LMO1 cell line 6812/2 was stably transfected with either the human ICN1 (intracellular NOTCH1) vector or empty vector (pcDNA3, Invitrogen, Carlsbad, CA). RT-PCR indicated that clones ICN2, ICN4, ICN5, and ICN6 expressed hICN1 mRNA, whereas clones ICN1 and ICN3 expressed little or no hICN1 mRNA. (C) Clones ICN1, 2, 4, and pcDNA1 (transfected with empty pcDNA3 vector) were treated with 5 µM Z-IL-CHO for 72 hours. (Upper panel) Western blot showing continued expression of ICN1 in stable transfectants treated with GSI. (Lower panel) Clones ICN1 and pcDNA1, which did not express hICN1, remained sensitive to the GSI, whereas clones ICN2 and ICN4 were no longer sensitive (* p < 0.01).
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