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Blood, Vol. 107, Issue 4, 1665-1672, February 15, 2006
"Maturational" globin switching in primary primitive erythroid cells
Blood Kingsley et al.
107: 1665
Supplemental materials for: Kingsley et al
Files in this Data Supplement:
- Table S1. Primers for PCR amplification of mouse genomic sequences (PDF, 37.7 KB)
- Figure S1. Agarose gel electrophoresis of sheared DNA for chromatin immunoprecipitation (ChIP) (JPG, 51.8 KB)
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After shearing by sonication, input DNA from a representative ChIP assay was subjected to electrophoresis on a 1.5% agarose gel. Nucleic acids are visualized by the inclusion of 1 microgram ethidium bromide/ml gel. Standard lane contains 100 bp ladder (Invitrogen). The majority of sheared DNA migrates between 300-700 base pairs.
- Figure S2. Separation by FACS of nucleated and enucleated erythroid cells from E12.5 peripheral blood (JPG, 99.1 KB)
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Ter119+, live (propidium iodide excluding) were analyzed for RNA (thiazole orange) and DNA (Hoescht) content.
- Figure S3. Histone modifications within the β-globin locus in primary primitive erythroid cells (E12.5) (JPG, 68.5 KB)
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The  -globin locus is represented to scale at the top, with the active globin genes indicated by arrows and exons of these genes by the thickest portions of the line. Unlabeled, thicker lines represent the positions of -globin pseudogenes. The positions of PCR-amplified regions analyzed in the ChIP assay are indicated immediately below. Shown in bar graph format are enrichments, relative to multiple control sequences derived from loci containing genes inactive in erythroid cells, of crosslinked chromatin samples immunoprecipitated using antiserum against histone H3 acetylated at lysines 9 and/or 16 (filled bars) or against histone H3 dimethylated at lysine 4 (empty bars). The border between shaded and unshaded regions in each graph represents relative enrichment of 1.0-fold (arrowhead). Values for enrichment greater than 20-fold have been cut off in this representation.
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