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Blood, Vol. 107, Issue 7, 2806-2813, April 1, 2006
Vaccination of human subjects expands both specific and bystander memory T cells but antibody production remains vaccine specific
Blood Genova et al.
107: 2806
Supplemental materials for: Di Genova et al
Files in this Data Supplement:
- Figure S1. Stable immunophenotypic profile of blood cells during the antigen-specific responses to vaccination with TT (JPG, 138 KB)
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(A) Kinetics of IFN- , IL-2 and IL-13 antigen-specific responses against TT, PPD and C. alb in subject 12 vaccinated with TT as measured by ELISPOT. Data for each time point (0, 1, 2 and 4 weeks) represent the mean number of spots ± SD of triplicate wells of antigen-stimulated cultures after subtracting the mean number of spots in the corresponding negative control. The mean numbers of spots in the controls were always lower then 5 spots per 2 × 105 PBMNC. (B) Blood counts and phenotype of immunological relevant cell subsets at the corresponding time points. During the antigen-specific response (A), no significant changes were found in the number of total white cells, or in the number of lymphocytes and monocytes in the blood (B). No significant changes were found in the percentages of CD3+CD4+ T lymphocytes, known to be the major population of cells responding in vitro to TT and PPD21,22 and in the proportion of CD14+ monocytes which constitute together with CD19+ B cells the major populations of antigen presenting cells within the PBMNC. Expression of the activation marker CD25+ within the total PBMNC and within the CD3+ lymphocytes population was checked and found to be unchanged (B). The level of MHC and co-stimulatory molecules was also measured and no change in the total percentages of cells expressing HLA-DR or the co-stimulatory molecule CD86 was detected (B). Finally, no significant changes were found in the percentages of T regulatory cells defined here as CD3+, CD4+, CD25High, CD45ROHigh (B).
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