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Blood, Vol. 107, Issue 11, 4407-4416, June 1, 2006
Global transcriptional coactivators CREB-binding protein and p300 are highly essential collectively but not individually in peripheral B cells
Blood Xu et al.
107: 4407
Supplemental materials for: Xu et al
Files in this Data Supplement:
- Table S1. Thirty probesets upregulated ≥ 1.7-fold in CBP null B-cells (PDF, 28.4 KB) -
Microarray analysis was performed using Affymetrix U74Av2 chips and RNA isolated from unstimulated splenic B-cells purified by positive selection. Average signal intensity for CBPflox/flox;CD19+/Cre (Mutants, n=2) and combined control mice [CBPflox/flox (n=2) and CBP+/flox;CD19+/Cre(n=2)]. Probesets shown had ≥ 1.7-fold higher average signal intensity in the mutants compared to combined controls. P < 0.05, two-tailed t-test. Probesets scored as absent in all six samples by the Affymetrix analysis software were excluded. Ratio of mutant average signal to control average signal indicated.
- Figure S1. Reduced expression of B220 in CBP null B-cell progenitors in the bone marrow (JPG, 94.6 KB)
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A. Hisograms of B220 FACS analysis in the gated lymphoid population of bone marrow and spleen. Peak B220 intensity of CD19+/Cre control cells is indicated by a vertical line. B. B220 mean fluorescence intensity (MFI) of pre-B (B220lo CD43— IgM—), immature B (B220int CD43— IgM+), and mature recirculating B (B220lo CD43— IgM+) cells derived from FACS analysis of bone marrow. Number of mice of each genotype indicated, mean ± s.e.m. Variation in absolute fluorescence between experiments leads to data scatter. Real-time RT-PCR analysis of B220 C and Rag1 control D in FACS-purified pre-B and immature B cell as in B. mRNA normalized to  -actin nRNA. Number of mice of each genotype indicated, mean ± s.e.m.
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