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Blood, Vol. 107, Issue 6, 2400-2408, March 15, 2006
Patterns of expression, membrane localization, and effects of ectopic expression suggest a function for MS4a4B, a CD20 homolog in Th1 T cells
Blood Xu et al.
107: 2400
Supplemental materials for: Xu et al
Files in this Data Supplement:
- Table S1. Representation difference analysis (RDA) of genes whose expression is regulated during T lineage commitment (PDF, 137 KB) -
Fluorescence-activated cell sorting was used to simultaneously isolate pre-committed CD44+ CD25—CD4—8— thymocytes and the post-committed CD25+CD44—CD4—8— thymocyte populations. A cDNA library was made by PCR subtraction (representation difference analysis) cloning using mRNA isolated from FACS-isolated CD44+/CD25—CD4—8— and CD44+ CD25—CD4—8— thymocytes of RAG-1—/— mice. The library was screened using probes generated by subtraction of CD44— CD25+ thymocyte RNA from CD44+ CD25— thymocyte RNA. The unique clones isolated based on their differential expression in the two subpopulations were re-tested for differential expression using independently amplified, RDA subtracted probes.
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