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Blood, Vol. 107, Issue 5, 2170-2179, March 1, 2006
The Polycomb group gene Ezh2 prevents hematopoietic stem cell exhaustion
Blood Kamminga et al.
107: 2170
Supplemental materials for: Kamminga et al
Files in this Data Supplement:
- Table S1. Genes that were higher expressed in MEFs passage 1 than in MEFs passage 5
(PDF, 54.1 KB) -
Total RNA was isolated from passage 1 (young) and passage 5 (aged) MEFs derived from C57BL/6 (B6) and DBA/2 (D2) mice with a RNeasy mini kit (Qiagen, Venlo, the Netherlands). During cDNA synthesis, cDNA samples were labeled with [33P]-dCTP (deoxycytidine triphosphate, MP Biochemicals, Irvine, Calif., USA). Two mouse filter gene arrays (GF400a, Research Genetics, Invitrogen, Breda, the Netherlands), each containing 5184 cDNA fragments, were hybridized with young and aged B6 and D2 cDNA samples as described previously (de Haan et al., 2002). Hybridization signals were detected using a Storm phospho imager system (Amersham Biosciences, Buckinghamshire, UK) and data were further analyzed using Pathways™ software (Research Genetics, Invitrogen, Breda, the Netherlands).
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