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Blood, Vol. 107, Issue 7, 2993-3001, April 1, 2006
Donor CD4+ T and B cells in transplants induce chronic graft-versus-host disease with autoimmune manifestations
Blood Zhang et al.
107: 2993
Supplemental materials for: Zhang et al
Files in this Data Supplement:
- Figure S1. Donor B cells induced expansion of donor CD4+ T cells that augmented anti-dsDNA secretion by donor B cells (JPG, 75.6 KB)
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A. CD4+ T cells (0.5 × 106) from chronic GVHD recipients given whole DBA/2 donor spleen cells (CD4+ T1) or from chronic GVHD-free recipients given donor B cell-depleted spleen cells (CD4+ T2) were added to cultures of B220+ B cells (1 × 106) from the spleen of the chronic GVHD recipients for 5 days. IgM and IgG anti-dsDNA autoantibody concentrations in the culture supernatant were measured by ELISA. Mean ± SE of IgM anti-dsDNA of 4 replicate experiments is shown, and IgG anti-dsDNA was not detectable. B.CD4+ T1 or T2 cells from above chronic GVHD or GVHD-free BALB/c recipients were co-injected with CD4+ T-depleted spleen cells from chronic GVHD recipients into sublethally irradiated BALB/c recipients. Seven days after cell injection, levels of serum IgG anti-dsDNA were measured with ELISA. There were 12 recipients in each group combined from two replicate experiments. Mean ± SE of 12 recipients is shown.
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