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Blood, Vol. 107, Issue 11, 4424-4432, June 1, 2006 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Foxp3+CD4+CD25+ T cells control virus-specific memory T cells in chimpanzees that recovered from hepatitis C Blood Manigold et al. 107: 4424 Supplemental materials for: Manigold et al Files in this Data Supplement: Figure S1. Addition of neutralizing TGF-β1 antibodies did neither revert the suppressor activity nor the anergic state of CD4+CD25+ T cells in any of the chimpanzees (JPG, 60.2 KB) - Data in Fig. 3A serve as the control (suppressor activity and proliferation of CD4+CD25+ T cells in the absence of neutralizing TGF-1 antibodies). Figure S2. Depletion of CD25+ T cells from PBMC (JPG, 80.4 KB) - (A) PBMC before (left panel) and after (right panel) magnetic depletion of CD25+ T cells. (B) Magnetic depletion did not profoundly alter the ratio of the remaining PBMC subpopulations. The panel on the right shows the average of the results of 5 HCV-recovered chimpanzees. Figure S3 (JPG, 75.2 KB) - (A) CD25+ T cells suppressed IFN- secretion of CD4+CD25— T cells to recombinant HCV-NS5A protein. SFC, spot forming cells. (B) CD4+CD25+ TRegs of the HCV-recovered chimpanzee 1605 inhibited IFN- secretion of autologous E1-, NS5B and NS3 peptide-specific CD8+ T cells, did not produce IFN- and did not require CD4+CD25— cells to exert suppressive function. The data are the mean of duplicate or triplicate cultures. APC, antigen-presenting cells (irradiated, CD4- and CD8-depleted PBMC). This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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