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Blood, Vol. 107, Issue 7, 2643-2652, April 1, 2006
Differentiation of naive cord-blood T cells into CD19-specific cytolytic effectors for posttransplantation adoptive immunotherapy
Blood Serrano et al.
107: 2643
Supplemental materials for: Serrano et al
Files in this Data Supplement:
- Figure S1. Supplement to Figure 5 (JPG, 249 KB)
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- Video 1. Time lapse microscopy of UCB-derived T cells killing CD19+ tumor cells (Supplement to Figure 5) (MOV, 1.53 MB)
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(A) Time lapse video frames at 5 minute intervals (time marks at top left corners) at 2× of the 320 × 240 pixel region corresponding to part II video described in part B (Video 1). Tumor cell killing (red “k”) and divisions (green “d”) are marked. Time points 0 and 55 minutes are presented in Figure 5D. (B) Video time lapse microscopy of CD19+ adherent U251T tumor cells being killed by an umbilical cord blood-derived CD19-specific CD8+ T-cell clone. The movie is divided into two parts with the elapsed minutes shown in the upper left corner. The first part provides an overview of the wide-spread killing of tumor cells by T cells. As shown in Figure 5B, most of the killing occurs within the first 60 minutes. The second part zooms in on an area of interest and tumor kill (red “k”) and divisions (green “d”) are marked in every frame. Part I of the movie is at 1× zoom of first 120 minutes of co-culture seen at 60× playback (640 × 480 pixels). Part II of the movie is at 2× zoom of first 60 minutes of coculture at 30× playback speed (320 × 240 pixels).
- Video 2. Time lapse images of tumor growth in mice undergoing adoptive immunotherapy (Supplement to Figure 6) (MOV, 5.99 MB)
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Movie (file Fig6video2) of time lapse BLI of ffLuc+ Daudi in two NOD/scid mice starting the day before adoptive immunotherapy and ending after 11 days. One mouse received adoptive transfer of CD8+ CD19-specific UCB-derived T-cell clone (left) and one mouse received no cellular therapy. Color bar displays relative ffLuc activity in units of p/s/cm2/sr.
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