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Blood, Vol. 107, Issue 8, 3173-3180, April 15, 2006
Fetomaternal cross talk in the placental vascular bed: control of coagulation by trophoblast cells
Blood Sood et al.
107: 3173
Supplemental materials for: Sood et al
Files in this Data Supplement:
- Figure S1 (PDF, 20.2 KB) -
Changes in expression of TF, TFPI and TM transcripts upon differentiation of TS to DT cells, measured by real time PCR. Transcript abundance was normalized to GAPDH and expressed as fold change over average abundance in TS cells. Statistically significant (P<0.05) fold changes are indicated (*).
- Figure S2 (PDF, 60.6 KB) -
Coagulation related gene expression in human choriocarcinoma cell lines. Expression status of coagulation related genes was assessed in BeWo and Jeg3 choriocarcinoma cells by RT-PCR (panel A). Both BeWo and Jeg3 cells responded with calcium signals upon stimulation with agonist peptides for Par1 (TRAP1, TFLLRNPND-NH2) and Par2 (TRAP2; SLIGRL-NH2) (panel B). Stimulation of Jeg3 cells with Par1 agonist peptide for 45 minutes resulted in a 13-fold increase in Egr1 and a 5-fold increase in Cyr61 expression, as determined by real time PCR (panel C). Statistically significant (P<0.05) fold changes are indicated by an asterix (*).
- Table S1 (PDF, 95.8 KB) -
Transcriptome changes in TS and DT cells in response to 90 minute treatment with Par1 and Par2 agonist peptides are provided as lists of up-regulated and down-regulated genes.
- Table S2 (XLS, 40 KB)
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Table lists expression data on coagulation related probe sets in TS and DT cells. Columns labeled TS1, TS2 etc and DT1, DT2 etc are signal intensities from individual expression profiles on TS and DT cells, respectively. TS1-d, DT1-d etc are detection calls determined by the DATA MINING TOOL algorithm for each experiment. Avg-TS and Avg-DT are average signal intensities, and Avg-TS-d and Avg-DT-d are aggregate detection calls assigned to each gene using the criteria described in methods.
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