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Blood, Vol. 107, Issue 11, 4417-4423, June 1, 2006
Interferon- –treated dendritic cells specifically induce proliferation of FOXP3-expressing suppressor T cells
Blood Mennechet and Uzé
107: 4417
Supplemental materials for: Mennechet and Uzé
Files in this Data Supplement:
- DCs treated with 100 pM IFNλ1 behave similarly to DCs treated with 3 nM for MHC surface expression and allogeneic reaction (PDF, 30.6 KB) -
iDCs were left untreated or treated with IFN at various concentrations for 24 hrs. (A) Surface expression of MHC Class I and MHC Class II were measured by flow cytometry. Results are mean of 2 different blood donors. (B) DCs were used for stimulating allogeneic CD4+ T-cell proliferation in vitro at 1/10 DC/T cells ratio. Neutralizing anti-IL-2 or control IgG Abs were added to the cultures as indicated. CD4+ T-cell proliferation was determined by [3H]-thymidine incorporation. (C) Quantification of Foxp3 mRNA expression was determined by real time PCR in MLR cultures mixing the indicated DCs and CD4+ T cells. Experiments were performed on a pool of T cells from 3 different blood donors. Results are expressed relatively to GAPDH level.
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