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Blood, Vol. 108, Issue 2, 609-617, July 15, 2006
ICSBP/IRF-8 differentially regulates antigen uptake during dendritic-cell development and affects antigen presentation to CD4+ T cells Files in this Data Supplement: Splenic DC from ICSBP—/— and WT mice were incubated with OVA peptide (323-339) for 30 min. at 37°C, then washed. OVA peptide-loaded s-DC were then cultured with OVA-specific OT-II CD4+ T cells. Proliferation was measured by pulsing the plates with (3H)-thymidine for 10 hours at the third day of culture. Thymidine incorporation into the cellular DNA was determined by liquid scintillation counting. Each point represents the mean cpm ± SD in triplicate cultures of one out of three experiments.
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