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Blood, Vol. 108, Issue 3, 1037-1044, August 1, 2006
Diagnostic application of flow cytometric characteristics of CD34+ cells in low-grade myelodysplastic syndromes
Blood Ogata et al.
108: 1037
Supplemental materials for: Ogata et al, Blood, Vol. 108, Issue 3, 1037-1044
Files in this Data Supplement:
- Figure S1. Reliability of gating of hematogones (PDF, 1.15 MB) -
(A-B) Confirmation of hematogone gating using 3-color FCM in preliminary experiments. In preliminary experiments, we performed 3-color FCM in which every cell sample was stained for CD45, CD34, and various third antigens, such as CD10 and CD33. We confirmed that when CD34+ cells were displayed on a CD45 versus SSC plot, stage I hematogones (CD34+CD10+CD19+ cells) formed a cluster at the left lower corner of CD34+ cells. This finding was reported previously by others18 and us.23 (A) CD45 versus SSC display of cells. CD34+ cells are green dots. (B) Immunophenotype of gated cells in panel A shows that CD34+ cells in this gate were CD10+CD19+CD13–CD33–. (C-F) For every patient and control in the present study, we confirmed the reliability of hematogone gating. A portion of cell events (usually 2-3 × 104 cells) was displayed on a CD45 versus SSC plot; CD34+ cells were green (D). This enabled easier detection of stage I hematogone clusters compared with the display of all cell events (more than 1 × 105 cells) (C). We made gates for stage I hematogones and myeloblasts (D) and confirmed the reliability of these gates by determining the phenotypes of the gated cell populations. (E) CD34+ cells in the myeloblast gate expressed myeloid antigens but not CD10. (F) CD34+ cells in the stage I hematogone gate expressed CD10 but not myeloid antigens.
- Table S1. Characteristics and results of diagnostic FCM in all LGw/oRS patients (XLS, 24 KB)
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